Benefits of Humaneering® Antibodies
This process for engineering antibodies results in sequences very close and in some cases identical to human germline. The property of closeness-to-human-germline (and not merely 'fully human') has implications beyond immunogenicity. Unlike other technologies, Humaneering® yields large panels of antibodies with the identical binding specificity; therefore, clones can be chosen that fulfill not only classic issues of antibody engineering (e.g., affinity and specificity), but also downstream processing issues (e.g., expression level, stability, etc.) as shown below:
V-region Sequences
- High amino-acid sequence identity with human germ-line, including CDRs 1+2 from each chain
- V-regions sequences are mainly from a human source and are close to germline in sequence
- CDR lengths and sequences are 'natural'
- Important for low immunogenicity
Affinity
- Binding affinity and selectivity is high
- Kinetics on/off rates can be selected
- Important for potency/dose/cost of goods
Expression Level
- Antibodies are screened for high level expression
- Important for cost of goods
Physicochemical Properties
- Mainly determined by V-region sequences
- Solubility, stability and lack of aggregation
- Important for low immunogenicity, cost of goods and route of delivery
Pipeline
KaloBios' pipeline is comprised of first or best-in-class antibody therapeutic candidates for cancers.